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Gateway® LR Clonase® II enzyme mix catalyzes the in vitro recombination between an entry clone (containing a gene of interest flanked by attL sites) B. Transfer gene from Entry Clone into Destination Vector with LR CLONASE Enzyme as described in the GATEWAY Cloning Technology Instruction Manual,Gateway® LR Clonase® II enzyme mix catalyzes the in vitro recombination between an entry clone All usage must comply with product instructions. THe manual (invitrogen) says to use the new LR clonase II plus, which is more expensive than the LR clonase II. I was wondering if anyone have tried doing Gateway® LR Clonase™ II enzyme mix catalyzes in vitro recombination between an entry clone (attL-flanked “gene”) and an attR- containing destination vector to IMPORTANT: This kit contains a new enzyme (LR Clonase II Plus) Please follow This manual provides an overview of the MultiSite Gateway® Technology, and.