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Antibiotic HiVeg Assay Media are used in the microbiological assay of different antibiotics in pharmaceutical products and also in foods, etc. Inhibition of Staphylococcus aureus ATCC 29737 by different concentrations of Cephalexin. 3 R Selection of Media and Test Organism for Assay of Antibiotics A N T I B I O T I C M E D I A F O R Residues of two antibacterial agents, cephalexin and colistin, co-administered by intramuscular injection to calves, were quantified in four different… Fluorimetric assay of cephradine, cephalexin and cephaloglycin. Barbhaiya, RH; Turner, P British Journal of Clinical Pharmacology, Volume 4 (4) - Aug 1, 1977 Read Article Download PDF Share Full Text for Free (beta) 5 pages Article Details Recommended References Bookmark Add to Folder Cite Social Times Cited: Web of Science Journals / Purchase Theory and application of Microbiological Assay - 1st Edition. Print Book & E-Book. ISBN 9780123464453, 9780323155281. Skip to content. (PDF) eBook Format Help. Add to cart. Sales tax will be 9.13 Cephalexin 9.14 Cephaloridine 9.15 Cephalothin 9.16 Chlortetracycline 9.17 Cloxacillin Cephalexin plasma concentration was determined by microbiological assay (Bennet et al.,1966) using Kocuria rhizophila, formerly classified as Micrococcus luteus (American Type Culture Collection 9341) as test microorganism. Each sample was measured in triplicate and a standard curve was prepared using normal rabbit plasma. from 0 to 6h (cephalexin) or 0 to 24 h (co-trimoxazole). After centrifugation, the plasma samples and aliquots from the measured urine collections were stored at —20°C, until analysis. The content of cephalexin in each sample was measured by a modification of the small plate microbiological assay technique of Grove & Randall (1955), using Antibiotic HiVeg Assay Medium No. 2 (Base HiVeg Agar) is used for microbiological assay of antibiotics. Composition** Ingredients Gms / Litre HiVeg peptone 6.000 HiVeg extract 1.500 Yeast extract 3.000 Agar 15.000 Cephalexin, Cephalothin, Cephapirin, Cloxacillin, Dicloxacillin, Methicillin, Nafcillin, Oxacillin, Chloramphenicol, The system was tested by assaying cephalexin with the aid of Bacillus subtilis in an FDA single‐dose design modified by reducing the number of standards to two. The influence of the thickness of the base layer and the form of the dose‐response line were tested. Zone diameter was measured with a resolution of 0.01 mm. vancomycin is based on microbiological assay (3). Due to its increased clinical significance, a number of high performance liquid chromatographic meth-ods for the determination of vancomycin in biologi-cal fluids have been developed (4-9). Due to the vital significance of vancomycin, the development of a simple and fast method for its Using microbiological assay method, the lowest concentration of cefoxitin which can be detected both in aqueous solution and serum was 2-5 ug/ml. There was a good correlation between the fluorimetric assay and the microbiological assay (microbiological: fluorimetric (found), y = O-9x+OO8, r = 0-99). The recovery Antibi