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Trim Galore! is a wrapper script to automate quality and adapter trimming as well as quality control, with some added functionality to remove biasedTrim Galore User Guide V0.4.0. User Manual: Open the PDF directly: View PDF PDF . Page Count: 12. 147528. May 06, 2015. Taking appropriate QC measures for. Trimgalore, cutadapt and prinseq have higher levels of mismatches along the length of the read. Flexbar has the worst level of mismatch frequency. In the Trim Galore! is a wrapper script to automate quality and adapter trimming as Updated User Guide and Readme documents, added Installation instruction and NAME¶. trim_galore - automate quality and adapter trimming for DNA sequencing. DESCRIPTION¶. USAGE: trim_galore [options] .